Dr Tom Sheard
School of Biosciences
Research Associate
T.Sheard@sheffield.ac.uk
Firth Court
Full contact details
Dr Tom Sheard
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
- Profile
-
Career history
- 2021-present – Postdoctoral research associate, School of Biosciences, University of Sheffield, UK
- 2016-2021 – PhD, Faculty of Biological Sciences, University of Leeds, UK
- 2015-2016 – MPhil, Faculty of Biology, Medicine and Health, University of Manchester, UK
- Research interests
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Super-resolution microscopy imaging techniques allow scientists to see nanoscale structures. These techniques often require specialist equipment and knowledge, rendering them inaccessible to a significant number of scientists in the field.In my post-doctoral research position I will develop accessible imaging techniques, making use of the novel super-resolution technique called expansion microscopy (ExM), which uses expanding hydrogels to physically inflate a sample to increase the resolution of imaging.I will innovate the ExM methodology, investigating new gel chemistries, fluorescent probes, and understanding the even-ness (isotropy) of expansion with calibration structures.I am interested in implementing ExM in novel applications which will benefit from improved resolution to see biological targets such as proteins, lipids and genetic material.
- Publications
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Journal articles
- Correction: Expansion microscopy reveals subdomains inC. elegansgerm granules. Life Science Alliance, 7(1), e202302405-e202302405.
- Geometry-preserving expansion microscopy microplates enable high-fidelity nanoscale distortion mapping. Cell Reports Physical Science, 101719-101719.
- Expansion microscopy reveals subdomains inC. elegansgerm granules. Life Science Alliance, 6(4), e202201650-e202201650.
- A correlative super-resolution protocol to visualise structural underpinnings of fast second-messenger signalling in primary cell types. Methods, 193, 27-37.
- Three-dimensional and chemical mapping of intracellular signaling nanodomains in health and disease with enhanced expansion microscopy. ACS Nano, 13(2), 2143-2157. View this article in WRRO
- 3D ultrastructural organisation of calcium release units in the avian sarcoplasmic reticulum. Journal of Experimental Biology, 222(7).
- Super-resolution analysis of the origins of the elementary events of ER calcium release in dorsal root ganglion neurons. Cells.
- Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy. Nanoscale.
- Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease. Royal Society Open Biology.
- Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis. Analytical Chemistry.
- 3D visualisation of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads. Philosophical transactions of the Royal Society of London. Series B, Biological sciences.
- Right-ventricular heart failure increases spatial heterogeneity in the sub-cellular expression of SERCA2a, reducing calcium-induced-calcium-release, increasing inter-cellular variability, and modulating vulnerability to pro-arrhythmogenic cellular phenomena. Philosophical transactions of the Royal Society of London. Series B, Biological sciences.
- Peptide ligands of the cardiac ryanodine receptor as super-resolution imaging probes. View this article in WRRO
- Molecular-scale 3D visualisation of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads.
Chapters
- Enhanced expansion microscopy to measure nanoscale structural and biochemical remodeling in single cells, Methods in Cell Biology (pp. 147-180). Elsevier
Conference proceedings papers
- Diverse labelling of cellular compartments with NHS esters in expansion microscopy. Biophysical Journal, Vol. 122(3) (pp 275a-275a)
- Super resolution techniques combined with image spatial correlation analysis to study Angiotensin II type 1 receptor' dynamic and organization in renal cells. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol. 52(SUPPL 1) (pp S167-S167)
- Sub-cellular Heterogeneity in SERCA Determines Spatial Calcium Dynamics in Cardiomyocytes. BIOPHYSICAL JOURNAL, Vol. 118(3) (pp 172A-173A)
- Optical Superresolution Analysis of Intracellular Calcium Handling Proteins and Correlating Calcium Signal Morphology. BIOPHYSICAL JOURNAL, Vol. 118(3) (pp 172A-172A)
Preprints
- Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy, Cold Spring Harbor Laboratory.
- Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping, Cold Spring Harbor Laboratory.
- Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease, Cold Spring Harbor Laboratory.
- A super-resolution protocol to correlate structural underpinnings of fast second-messenger signalling in primary cell types, Cold Spring Harbor Laboratory.
- Expansion microscopy reveals subdomains in C. elegans germ granules, Cold Spring Harbor Laboratory.