Equipment
The Facility is based in a very well-equipped large molecular genetics laboratory. We also have a separate clean room which is used for the pre-PCR stages during ancient DNA work and BeadXpress-based SNP typing. A suite of PC computers are available. We also have a walk-in refridgerated 4C room, two -18C freezer rooms, numerous standalone -18C freezers and three -80C freezers.
| Some of the equipment available at the Facility includes: |
|
|
|
1.) A high-throughput Applied Biosystems 3730 48-capillary DNA analyzer, which is used for sequencing and the automated genotyping of microsatellites.
|
Our ABI 3730 48-well capillary DNA Analyzer
|
| We use a 4-dye Rox size standard set (with 6-FAM, NED, and HEX fluoro-labels available for markers) and the 5-dye LIZ size standard set (with 6-FAM, NED, VIC and PET). |
Multiplex genotype profile
|
| 2.) An Illumina BeadXpress SNP genotyping platform. |
Illumina BeadXpress Reader
|
| 3.) An Applied Biosystems 3100 16-well Capillary DNA Analyzer used for Reference Strand-mediated Conformation Analysis (RSCA). |
|
| 4.) An Applied Biosystems StepOneTM Real-time PCR system. |
Step-one real time PCR
|
| 5.) A Biomek 2000 robotic liquid-handling workstation with peripherals including a plate-reading fluorometer. |
|
| 6.) An Agilent Bioanalyzer. |
Agilent Bioanalyzer
|
| 7.) A Nanodrop 8000 and a fluorimeter and spectrophometer for assessing the concentation of DNA and RNA. |
Nanodrop 8000
|
|
|
|
