Techniques supported

We are open to supporting a wide range of techniques.

Examples of the services offered include:

Metabarcoding (e.g. for studies of eDNA, diet analysis, parasite screening)
SNP marker development and typing
Microsatellite marker development and typing
High-throughput DNA extraction
Quantitative PCR (including telomere length analysis)
Genotyping by sequencing
MHC genotyping
PCR sex-typing

We offer analytical support for all the techniques taught.

Researchers visit Sheffield to use the facilities under the supervision of experienced staff. This provides an ideal forum for discussion and support of projects.

Projects that contribute to the training of research students or others are encouraged.

We commonly support projects involving metabarcoding, microsatellite isolation, microsatellite genotyping, SNP-typing and sex-typing. Training and facilities for the application of microsatellite marker sets for automated genotyping has historically been the core request of the Facility (see Supported Publications).

The Facility is also able to support other applications, including Amplified Fragment Length Polymorphism analysis (AFLP), Reference Strand-mediated Conformation Analysis (RSCA), multi-locus and single-locus minisatellite DNA fingerprinting.

 We are very willing to consider extending the range of techniques supported, please contact Dr Deborah Dawson (d.a.dawson@sheffield.ac.uk) to discuss.