The University of Sheffield
Department of Molecular Biology and Biotechnology

Selected Publications


1. PITTMAN, M. S., ELVERS, K. T., LEE, L., JONES, M. A., POOLE, R. K., PARK, S. F. & KELLY, D. J. (2007) Growth of Campylobacter jejuni on nitrate and nitrite: electron transport to NapA and NrfA via NrfH and distinct roles for NrfA and the globin Cgb in protection against nitrosative stress. Mol. Microbiol. 63, 575-590.
2. JACKSON, R. J., ELVERS, K. T., LEE, L. J., GIDLEY, M. D., WAINWRIGHT, L. M., LIGHTFOOT, J., PARK, S. F. & POOLE, R. K. (2007) Oxygen reactivity of both respiratory oxidases in Campylobacter jejuni: the “cydAB” genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd-type. J. Bacteriol. 189, 1604-16
3. PARTRIDGE, J. D., SANGUINETTI, G., DIBDEN, D., ROBERTS, R. E., POOLE, R. K. & GREEN, J. (2007) Transition of Escherichia coli from aerobic to micro-aerobic conditions involves fast and slow reacting regulatory components. J. Biol. Chem. 282, 11230-11237
4. PULLAN, S. T., GIDLEY, M. D., JONES, R. A., BARRETT, J., STEVANIN, T. M., READ, R. C., GREEN, J. & POOLE, R. K. (2007) Nitric Oxide in Chemostat-Cultured Escherichia coli is Sensed by Fnr and Other Global Regulators; Unaltered Methionine Biosynthesis Indicates Lack of S-Nitrosation. J. Bacteriol. 189, 1845-1855.
5. PARTRIDGE, J. D., POOLE, R. K. & GREEN, J. (2007) The Escherichia coli yhjA gene, encoding a predicted cytochrome c peroxidase, is regulated by FNR and OxyR. Microbiology 153, 1499-1507.
6. GILBERTHORPE, N. J., LEE, M. E., STEVANIN, T. M., READ, R. C. & POOLE, R. K. (2007) NsrR: a key regulator circumventing Salmonella enterica serovar Typhimurium oxidative and nitrosative stress in vitro and in IFN-g-stimulated macrophages. Microbiology 153, 1756-1771
7. STEVANIN, T. M., LAVER, J. R., POOLE, R. K., MOIR, J. W. B. & READ, R. C. (2007) Metabolism of nitric oxide by Neisseria meningitidis modifies release of NO-regulated cytokines and chemokines by human macrophages. Microb. Infect., 9, 981-987.
8. LU, C., EGAWA, T., WAINWRIGHT, L. M., POOLE, R. K. & YEH, S.-R. (2007) Structural and functional properties of a truncated hemoglobin from a food-borne pathogen Campylobacter jejuni. J. Biol. Chem. 282, 13627-13636.
9. STEVANIN, T. M., READ, R. C. & POOLE, R. K. (2007) The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: links with swarming motility. Gene 398, 62-68.
10. SHEPHERD, M., HEATH, M. D. & POOLE, R. K. (2007) NikA binds heme: a new role for a periplasmic nickel-binding protein. Biochemistry 46, 5030-5037
11. LU, C., MUKAI, M., LIN, Y., WU, G., POOLE, R. K. & YEH, S.-R. (2007) Structural and functional properties of a single-domain hemoglobin from the food-borne pathogen Campylobacter jejuni. J. Biol. Chem. 282, 25917-25928
12. POOLE, R. K. (2008) (ed.) Advances in Microbial Physiology, vol. 53. 311 pp.. London: Elsevier Ltd
13. KALNENIEKS, U., GALININA, N., STRAZDINA, I., KRAVALE, Z., PICKFORD, J. L. RUTKIS, R. & POOLE, R. K. (2008) NADH dehydrogenase deficiency results in low respiration rates and improved aerobic growth of Zymomonas mobilis. Microbiology. 154, 989-994
14. LATIMER, J., STOKES, S., McLEOD, C. & POOLE, R. K. (2007) Gene expression and heterogeneity in bacterial communities. In: Biofilms: Coming of Age (eds. P. Gilbert, D. Allinson, M. Brading, J. Pratten, D. Spratt and M. Upton), pp. 69-78. The Biofilm Club, Manchester.
15. LEWIS, M. E. S, CORKER, H. A., GOLLAN, B. & POOLE, R. K. (2008) A survey of methods for the purification of microbial flavohemoglobins. In: Methods in Enzymology. vol. 436. Globins and Other NO-Reactive Proteins, Part A, pp. 169-186. San Diego: Elsevier.
16. PICKFORD, J. L., WAINWRIGHT, L, WU, G. & POOLE, R. K. (2008) Expression and purification of Cgb and Ctb, the NO-inducible globins of the foodborne bacterial pathogen Campylobacter jejuni. In: Methods in Enzymology. vol. 436. Globins and Other NO-Reactive Proteins, Part A, pp. 289-302. San Diego: Elsevier.
17. PULLAN, S. T., MONK, C. E., LEE, L. & POOLE, R. K. (2008) Microbial responses to nitric oxide and nitrosative stress: growth, 'omic' and physiological methods. In: Methods in Enzymology. vol. 437. Globins and Other NO-Reactive Proteins, Part B, pp. 499-519. San Diego: Elsevier.
18. LU, C., EGAWA, T., MUKAI, M., POOLE, R. K. & YEH, S.-R. (2008) Hemoglobins from Mycobacterium tuberculosis and Campylobacter jejuni: A comparative study with resonance Raman spectroscopy. In: Methods in Enzymology. vol. 437. Globins and Other NO-Reactive Proteins, Part B, pp. 255-286. San Diego: Elsevier.
19. PARTRIDGE, J. D., BROWNING, D. F., XU, M., NEWNHAM, L. J., SCOTT, C., ROBERTS, R. E., POOLE, R, K. & GREEN, J. (2008) Characterization of the Escherichia coli K-12 ydhYVWXUT operon: regulation by FNR, NarL and NarP. Microbiology 154, 608-618.
20. GRUIA, F., KUBO, M., YE, X., IONASCU, D., LU, C., POOLE, R. K., YEH, S.-R. & CHAMPION, P. (2008) Coherence spectroscopy investigations of the low-frequency vibrations of heme: Effects of protein-specific perturbations. J. Am. Chem. Soc. 130, 5231-5244.
21. GILBERTHORPE, N. J. & POOLE, R. K. (2008) Nitric oxide homeostasis in Salmonella typhimurium: roles of respiratory nitrate reductase and flavohemoglobin. J. Biol. Chem. 283, 11146-11154.
22. POOLE, R. K. (2008) Microbial haemoglobins – proteins at the crossroads of oxygen and nitric oxide metabolism. In: Dioxygen Binding and Sensing Proteins. A Tribute to Beatrice and Jonathan Wittenberg. (Bolognesi, M., Di Prisco, G. & Verde, C.). Springer-Verlag Italia
23. MONK, C. E., PEARSON, B. M., MULHOLLAND, F. & POOLE, R. K. (2008) Oxygen- and NssR-dependent globin expression and enhanced iron acquisition in the response of Campylobacter to nitrosative stress. J. Biol. Chem., 283, 28413-28425.
24. JACKSON, R. J., BINET, M. R. B., LEE, L. J., MA, R., GRAHAM, A. I., McLEOD, C. W. & POOLE, R. K. (2008) Expression of the PitA phosphate/metal transporter of Escherichia coli is responsive to zinc and inorganic phosphate levels. FEMS Microbiol. Lett. 289, 219-224.