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Professor Robert K. Poole
- Work in my laboratory is focussed on microbial physiology - the study of how bacteria and other microorganisms work. Although rooted in the tradition of bacterial growth and intermediary metabolism, microbial physiology now embraces molecular biology, genetics, biochemistry, and indeed any discipline that can shed light on bacterial function. Much of our experimental work is conducted with Escherichia coli, the pre-eminent 'model' organism with unrivalled ease of genetic and physiological manipulation. We are also work on its close relation, Salmonella (an intracellular pathogen and cause of enteritis), Campylobacter jejuni (the most common cause of food-borne disease), as well as Mycobacterium and Neisseria species. Special foci at present are bacterial respiratory chains and haemoglobins, nitrosative stress tolerance in bacteria, the biochemistry of CO interactions and oxygen metabolism.
- The approaches used to study these problems range from molecular genetics, through microbial biochemistry and biophysics, to physiology and the properties of microbial populations in vitro and in vivo. Consequently, the tools we use are diverse; they include manipulations in vitro and in vivo of nucleic acids, bacterial molecular genetics (e.g. microarray technology, transposon-mediated and site-directed mutagenesis, gene and operon fusions), protein purification, immunochemical methods and characterisation using optical (dual- and multi-wavelength spectrophotometry) tools.
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Lesley Bowman
I have recently graduated with a degree in Microbiology from the University of Glasgow. At present, I am working towards a PhD through research into the S-nitrosoproteome in enterobacteria and assessing cellular protective functions in vitro and in vivo.
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Kelly Davidge
I am post doctoral researcher currently working on an EC funded FP7 project, which aims to charaterise and investigate the role of a truncated globin, HbN, from Mycobacterium tuberculosis. We are testing the hypothesis that HbN has a partner reductase that aids in the detoxification of nitric oxide. I am also interested in the use of carbon monoxide-releasing molecules, or CO-RMs, as anti-bacterial agents against bacteria.
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Dr Alison Graham
I am a post-doctoral reseacher working on a SysMO-funded project entitled "Systems Understanding of Microbial Oxygen Responses" (SUMO). SUMO is a consortium that brings together biologists and modellers from all over Europe. The work investigates the responses of E. coli to oxygen availability, considering how the individual components that make up a biological system work together to produce coherent patterns of behaviour. We will collectively obtain and analyse highly reproducible data sets that will be integrated into elaborate predictive mathematical and computer science models in an iterative process of model-based hypothesis generation and experimental design. Understanding how the FNR and ArcA proteins sense oxygen will form a large part of the project.
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Mark Johnson
I am the Technician in the lab, maintaining the equipment and consumables.
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Dr Sam McLean
I am a post doctoral researcher investigating nitrosative stress in enterobacteria. My most recent work involves analysis of the transcriptome of E. coli and the S-nitrosoproteome of Salmonella upon exposure to peroxynitrite and S-nitrosoglutathione respectively.
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Carlos Avila Ramirez
I am a first year PhD student, my project is a Systems Biology approach of the response of Campylobacter jejuni to nitric oxide. I'm also investigating the kind of stress C. jejuni faces inside an avian host.
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Dr Mark Shepherd
I am a postdoctoral researcher investigating nitrosative stress in Campylobacter jejuni. My current work involves the biochemical analysis of NssR, a transcription factor that regulates the detoxification of nitric oxide.
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Holly Smith
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Jayne Louise Wilson
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