Dr Lynne Prince MSc PhD

Russell Fellow

Department of Infection and ImmunityDr Lynne Prince
L Floor
The University of Sheffield Medical School
Beech Hill Road
Sheffield
S10 2RX
UK

Tel:  +44 (0)114 271 2578
Fax: +44 (0)114 226 8898
Email: l.r.prince@sheffield.ac.uk

Biography

I graduated from Newcastle University in 1997 with a degree in medical microbiology. I then studied for the MSc in pathological sciences at the University of Sheffield (1999) which led to my PhD entitled `The regulation of neutrophil apoptosis by bacterial products´ (2003). In 2004 I was awarded a University of Sheffield Russell Research Fellowship to study the molecular mechanisms of neutrophil activation and apoptosis.  My scientific mentors are Professor Moira Whyte and Professor Ian Sabroe (Infection and Immunity).

Research Interests

Inflammation and inflammatory diseases such as chronic obstructive pulmonary disease, or rheumatoid arthritis are extremely difficult to treat effectively. During inflammation, inflammatory cells, in particular neutrophils and monocytes, are thought to be key mediators of both tissue damage and perpetuation of further inflammatory signalling.

My research focuses on the role of neutrophils during infection and inflammation, and how their inflammatory potential and function is regulated by apoptosis.  In particular, a collaboration with the University of Cardiff explores the cellular basis of inflammation in the preterm lung and partnerships with Professor Simon Foster and Dr Lynda Partridge (MBB) allow my group to study interactions between neutrophils and Staphylococcus aureus. Studying the roles of neutrophils in vivo is carried out in collaboration with Professor Steve Renshaw, using zebrafish models of inflammation (Infection and Immunity & MRC Centre for Developmental and Biomedical Genetics).

Professional activities

  • I am a member of the Society for Leukocyte Biology and the British Society for Immunology.

Current projects

  • 2014-2016 British Lung Foundation project grant to study "The regulation of inflammatory signalling in human macrophages in the context of COPD" (Dr Lynne Prince, Prof Ian Sabroe).
  • 2014-2016 Commonwealth Studentship to study "Dysregulated neutrophil function in COPD" (Dr Lynne Prince, Prof David Dockrell, Prof Moira Whyte).
  • 2013-2016 University of Sheffield Florey Studentship “Identifying novel immune modulating factors in a genome-wide S. aureus screen in primary human leukocytes” (Dr Lynne Prince, Prof Simon Foster, MBB).
  • 2013-2016 University of Sheffield Faculty studentship “The expression and roles of tetraspanins in human neutrophils” (Dr Lynne Prince, Dr Linda Partridge, MBB)
  • 2013-2014 University of Sheffield BMedSci studentship “Immune signaling in human macrophages, exploring roles for Pellino 1” (Dr Lynne Prince, Prof Ian Sabroe).
  • 2010-2013 MRC PhD studentship “The role of Hax1 in regulating myeloid cell survival in vitro and in vivo” (Dr Lynne Prince, Prof Steve Renshaw).

Key publications

  1. L.R. Prince, N.C. Maxwell, S.K. Gill, D.H. Dockrell, I. Sabroe, E.P. McGreal, S.Kotecha, M.K. Whyte.  Macrophage phenotype is associated with disease severity in preterm infants with chronic lung disease.  PLOS ONE, 2014, Aug.
  2. A. Basran, M. Jabeen, L. Bingle, C. A. Stokes, D. H. Dockrell, M. K. Whyte, S. R. Walmsley, K. R. Higgins, S. N. Vogel, H. L. Wilson, L. R. Prince, E. C. Prestwich, R. A. Sabroe, L. C. Parker, I. Sabroe. Roles of neutrophils in the regulation of the extent of human inflammation through delivery of IL-1 and clearance of chemokines. Journal of Leukocyte Biology, 2013, Jan;93(1):7-19.
  3. J. A. Bennett, L. R. Prince, L. C. Parker, C. A. Stokes, H. G. de Bruin, M. van den Berge, I. H. Heijink, M. K. Whyte, I. Sabroe. Journal of Virology, 2012, 86(12):6595-604.
  4. L. R. Prince, K. J. Graham, J. Connolly, S. Anwar, R. Ridley, I. Sabroe, S. J Foster, M. K. B. Whyte. Staphylococcus aureus Induces Eosinophil Cell Death Mediated by α-hemolysin. PLoS ONE, 2012, 7(2):e31506.
  5. L. R. Prince, M. K. Whyte, I. Sabroe, L. C. Parker. The role of TLRs in neutrophil activation. Curr Opin Pharm, 2011, 11(4):397-403.
  6. M. Uddin, G. Nong, J. Ward, G. Seumois, L. R. Prince, S. Wilson, G. Dent and R. Djukanović. Pro-survival activity for airway neutrophils in severe asthma. Thorax, 2010 65(8):684-9
  7. S. Anwar, L.R.Prince, S. J. Foster, M. K. B. Whyte and I. Sabroe. The rise and rise of Staphylococcus aureus: laughing in the face of granulocytes. Clin Exp Immunol, 2009, 157:216-224.
  8. E. P. Dick, L.R. Prince, E. Jones, S. A. Renshaw, M. K. B. Whyte, and I Sabroe. Pathways regulating LPS-induced Neutrophil Survival Revealed by Lentiviral Transduction of Primary Human Neutrophils. Immunology, 2008, , 127:249-255.
  9. E. P. Dick, L.R.Prince and I. Sabroe. Ex Vivo Expanded BM CD34+ Derived Neutrophils Have Limited Bactericidal Ability. Stem Cells, 2008, 26:2552-2563.
  10. L. R. Prince, S. M. Bianchi, K. Vaughan, M. B. Bewley, H. M. Marriott, S. R. Walmsley, G. W. Taylor, D. J. Buttle, I. Sabroe, D. H. Dockrell and M. K. B. Whyte. Subversion of a lysosomal pathway regulating neutrophil apoptosis by a major bacterial toxin, pyocyanin. J Immunol, 2008, 180(5):3502-11.
  11. S. M. Bianchi, L. R. Prince, K. McPhillips, L. Allen, H. M. Marriott, G. W. Taylor, P. G. Hellewell, I. Sabroe, D. H. Dockrell, P. W. Henson and M. K. B. Whyte. Impairment of apoptotic cell engulfment by pyocyanin, a toxic metabolite of Pseudomonas aeruginosa. Am J Respir Crit Care Med, 2008, 177(1):35-43.
  12. L. Parker, E. C. Jones, L. R. Prince, S. K. Dower, M. K. B. Whyte and I. Sabroe. Endotoxin Tolerance Induces Selective Alterations in Neutrophil Function. J Leukoc Biol, 2005, 78: 1301-1305.
  13. L. R. Prince, L. Allen, P. G Hellewell, S. K. Dower, M. K. B. Whyte and I. Sabroe. The Role of IL-1b in Toll-like Receptor 4-mediated Neutrophil Activation and Survival. Am J Pathol, 2004, 165:1819-1826.
  14. S. Kotecha, R. J. Mildner, L. R. Prince, J. R. Vyas, A. E. Currie, R. A. Lawson, M. K. B. Whyte. The Role of Neutrophil Apoptosis in the Resolution of Acute Lung Injury in Newborn Infants. Thorax, 2003, 5:961-7.
  15. I. Sabroe, L. R. Prince, E. C. Jones, M. J. Horsburgh, S. J. Foster, S. N. Vogel, S. K. Dower, and M. K. B Whyte. Selective Roles for TLR2 and TLR4 in the Regulation of Neutrophil Activation and Lifespan. J Immunol, 2003, 170, 5268-5275.
  16. L. R. Usher, R A. Lawson, I Geary, C J. Taylor, C D. Bingle, G W. Taylor, and M K.B. Whyte. Induction of Neutrophil Apoptosis by the Pseudomonas aeruginosa Exotoxin Pyocyanin: A Potential Mechanism of Persistent Infection. J Immunol, 2002, 168: 1861–1868.