Dr Colin D Bingle
Academic Unit of Respiratory Medicine
Department of Infection and Immunity
The University of Sheffield Medical School
Beech Hill Road
Tel: +44 (0)114 271 2423
Fax: +44 (0)114 271 3892
Secretary - Ms Pauline Whitaker
Tel: +44 (0)114 271 2027
Currently, I am a Reader in Respiratory Cell and Molecular Biology in the Academic Unit of Respiratory Medicine in the Department of Infection and Immunity at the University of Sheffield. I have been at Sheffield since 1997 when I was appointed to a Lectureship to support the development of molecular and cell biology within the Unit following the appointment of Professor Moira Whyte.
My research has been focused on lung epithelial cell biology since I undertook a Postdoctoral Fellowship at Washington University in St Louis between 1991 and 1993. During that time I became interested in the molecular basis of cell type specific gene expression and I published the first ever paper that identified a specific functional role for a transcription factor (HNF-3) in the regulation of a lung specific gene (Biochem J 1993;295:227-232). Since this time my work has continued to employ molecular biology techniques to address questions relating to the regulation, structure, expression and function of pulmonary epithelial genes.
My long-standing research interests have been focused on cellular differentiation and regulation of gene expression within the developing and adult pulmonary epithelium. This work involves the isolation of pulmonary epithelial cell specific genes as well as the transcription factors, which regulate their expression. Over the past few years my interests have expanded into the fields of the genetics of complex diseases, pulmonary immunology, innate immunity and host defence. Over my time in Sheffield I have also come to appreciate the importance of utilising clinical material as a scientific resource and I have established multiple collaborations to enable the collection and use of such samples for my work.
My teaching interests lie in aspects of genetics and comparative genomics which I teach on the MSc in Molecular Medicine. I also teach on the Undergraduate Medical Course
- I am Chair of The Biochemical Society Theme panel VI: Biological Systems and a member of the Meetings Board of the Biochemical Society.
- I am a member of the Governing Council of the Biochemical Society.
- I am a member of the Long Range Planning Committee of the Cell and Molecular Biology Assembly of the European Respiratory Society.
- I am Faculty representative on the University Health and Safety Committee and Chair of the Medical School Health and Safety Committee.
- I am on the Editorial Board of Experimental Lung Research and am an Editorial Advisor for the Biochemical Journal and Biochemical Society Transactions.
My research projects largely focus on the identification and characterisation of novel innate immune regulators, particularly members of the WFDC (Oncogene 2002;21:2768-2773) and PLUNC (Hum Mol Gen 2002;11:937-943) protein families. Indeed we identified the later family of proteins and they have gone on to become a significant area of my research focus. Laterly, I have become involved in studies designed to investigate the function and expression of these genes in the developing and diseased respiratory tract using a wide range of clinical samples (J Path 2005; 205: 491-497, Resp Res 2006; April 6;7:61, Resp Res 2007; Nov7:8:79).
My continuing and future studies in this area will elucidate the role these proteins play in a range of pulmonary disease including chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, cystic fibrosis (CF) and asthma in adults as well as in neonatal and paediatric diseases including RSV bronchiolitis and otitis media. I am also expanding my studies into the more tractable model systems with the aim of identifying the function of individual genes from these gene families.
I also work extensively with primary human airway epithelial cells in differentiated air liquid interface cultures as tools to understand the regulation of airway epithelial cell specific genes. At a more clinical level we are also studying host/pathogen interactions using both viral (RSV) and bacterial (Pseudomonas and NTHi) infections. We are presently using these models to understand the biology of co-infections with both RSV and NTHi.
I am using comparative genomic approaches to identify the molecular basis for the evolution of these host defence protein families. This work involves analysis and characterisation of gene families from a range of vertebrate species including chickens, cows and frogs as well as SNP analysis and studies of recent evolutionary events in the primate lineage.
- 2013-2016 £324,581 Biotechnology and Biological Sciences Research Council. The role of SPLUNC1/BPIFA1 in the host response to respiratory virus infection.
- 2012-2015 £52,000 University of Sheffield Research Studentship. The role of PLUNCs in otitis media.
- 2010-2013 £72,000 Sheffield Children's Hospital Trust. Modelling the airway epithelium in vitro as a tool for understanding pulmonary innate defence mechanisms.
- Musa M, Wilson K, Sun L, Mulay A, Bingle L, Marriott HM, LeClair EE, Bingle CD (2012). Differential localisation of BPIFA1 (SPLUNC1) and BPIFB1 (LPLUNC1) in the nasal and oral cavities of mice. Cell and Tissue Research Dec;350(3):455-64.
- Bingle L, Wilson K, Musa M, Araujo B, Rassl D, Wallace WA, LeClair EE, Mauad T, Zhou Z, Mall MA, Bingle CD (2012). BPIFB1 (LPLUNC1) is upregulated in cystic fibrosis lung disease. Histochemistry and Cell Biology 138:749–75.
- Shin OS, Uddin T, Citorik R, Wang JP, Della Pelle P, Kradin RL, Bingle CD, Bingle L, Camilli A, Bhuiyan TR, Shirin T, Ryan ET, Calderwood SB, Finberg RW, Qadri FQ, LaRocque RC, Harris JB (2011). LPLUNC1 Modulates Innate Immune Responses to Vibrio cholerae. Journal of Infectious Diseases 204(9):1349-5.
- Bingle CD (2011). Towards defining the complement of mammalian WFDC domain containing proteins. Biochemical Society Transactions Oct 1;39(5):1393-7.
- Bingle CD, Seal RL and Craven CJ (2011). Systemic nomenclature for the PLUNC/PSP/BSP30/SMGB proteins as a subfamily of the BPI foldcontaining superfamily. Biochem Soc Trans Aug 1; 39 (4): 977-83.
- Bingle CD, Wilson K, Lunn H, Barnes FA, High AS, Wallace WA, Rassl D, Campos MA, Ribeiro M, and Bingle L (2010). LPLUNC1, a secreted product of goblet cells and minor glands of the upper aero-digestive tract, Histochemistry and Cell Biology 133 (5) 505-15.
- Bingle L, Barnes FA, Lunn H, Musa M, Webster S, Douglas CWI, Cross SS, High AS, and Bingle CD (2009). Characterisation and expression of SPLUNC2, the human orthologue of rodent Parotid Secretory Protein. Histochemistry and Cell Biology 132(3):339-49.
- Bingle L, Barnes FA, Cross SC, Rassl D, Wallace WA, Campos MA, and Bingle CD (2007). Differential epithelial expression of the putative innate immune molecule, SPLUNC1 in Cystic Fibrosis. Respiratory Research November 7; 8:79.
- Bingle L, Cross SS, High AS, Wallace WA, Rassl D, Yuan G, Hellstrom I, Campos MA, and Bingle CD (2006). WFDC2 (HE4): a potential role in the innate immunity of the oral cavity and respiratory tract and the development of adenocarcinomas of the lung. Respir Res Apr 6;7:61.
- Bingle CD and Craven CJ. (2002) Plunc: A novel family of candidate host defence proteins expressed in the upper airways and nasopharynx. Human Mol Gen 11;937-943.