Dr. Lynne Bingle BSc (Hons), PhD
Lecturer
Tel: 0114 271 7953
Fax: 0114 271 7894
Email: l.bingle@sheffield.ac.uk
Research Interests
My main area of interest is in the PLUNC protein family which we hypothesise will play an important role in innate immunity. The expression of the PLUNCs appears to be restricted to regions of the lung, nose, mouth and female reproductive tract but the different family members exhibit very distinct expression patterns. We are presently comparing expression in normal and diseased tissues; this data alongside our functional studies will allow us to determine the precise the role of PLUNC proteins in host defence of the lung, nose and mouth.
In addition to my work on the PLUNC gene family I have a longstanding interest in a further group of host defence proteins, the whey acidic domain (WAP) protein family including the antiproteinases, SLPI and elafin. We have recently cloned and characterised the gene for a novel member of this family, HE4 (WFDC2), which had previously been shown to be expressed in both the male and female reproductive tracts and to be a potential marker for ovarian cancer. We have shown that HE4 is expressed in a range of lung cancers. As with the PLUNC proteins I am performing studies on these WAP proteins with a view to understanding how they may function in concert to protect the mucosal surfaces from pathogens.
Recently Published Papers
Bingle L, Cross SS, High AS, Wallace WA, Rassl D, Yuan G, Hellstrom I, Campos MA, Bingle CD (2006). WFDC2 (HE4): a potential role in the innate immunity of the oral cavity and respiratory tract and the development of adenocarcinomas of the lung. Respir Res. 7:61.
Bingle L, Lewis CE, Corke KP, Reed WR, and Brown NJ (2006). Macrophages promote tumour angiogenesis in human breast tumour spheroids in vitro. Brit J Cancer 94:101-107.
Ward JR, Bingle L, Judge HM, Brown SB, Storey RF, Whyte MKB, Dower SK, Buttle DJ and Sabroe I (2005). Agonists of Toll-like Receptor (TLR) 2 and TLR4 are Unable to Modulate Platelet Activation by Adenosine Diphosphate and Platelet Activating Factor. Thrombosis and Haemostasis 94:831-838.
Bingle L, Cross SS, High AS, Wallace WA, Devine DA, Havard S, Campos MA and Bingle CD (2005). SPLUNC1 (PLUNC) is expressed in glandular tissues of the respiratory tract and in cancers with a glandular phenotype. J. Pathol. 205:491-497.
Shi YH, Wang YX, Bingle L, Gong LH, Heng WJ, Li Y and Fang WG (2005). In vitro study of HIF-1 activation and VEGF release by bFGF in the T47D breast cancer cell line under normoxic conditions: involvement of PI-3K/Akt and MEK1/ERK pathways. J. Pathol. 205:530-536.
Geetha C, Venkatesh SG, Bingle L, Bingle CD and Gorr SU (2005). Design and validation of anti-inflammatory peptides from human Parotid Secretory Protein. J. Dental Res. 84:149-153.
LeClair EE, Nomellini V, Bahena M, Singleton V, Bingle L, Craven CJ and Bingle CD (2004). Cloning and expression of a mouse member of the PLUNC protein family exclusively expressed in tongue epithelium. Genomics 83:658-666.
Bingle CD, LeClair EE, Havard S, Bingle L, Gillingham P and Craven CJ (2004). Phylogenetic and evolutionary analysis of the PLUNC gene family. Protein Science 13:422-430.
Bingle L, Brown NJ and Lewis CE (2002). The role of tumour–associated macrophages in tumour progression: implications for new anti-cancer therapies. J. Pathol. 196:254-265.
Bingle L, Singleton V and Bingle CD (2002). The putative ovarian tumour marker gene HE4 (WFDC2) is expressed in normal tissues and undergoes complex alternative splicing to yield multiple protein isoforms. Oncogene 21:2768-2773.
Bingle L, Tetley TD and Bingle CD (2001). Cytokine mediated induction of the human Elafin gene in pulmonary epithelial cells is regulated by NF-?B. Am. J. Respir. Cell. Mol. Biol. 25:84-91.
LeClair EE, Nguyen L, Bingle L, MacGowan A, Singleton V, Ward SJ and Bingle CD (2001). Genomic organisation of the mouse plunc gene and expression in the developing airways and thymus. Biochem. Biophys. Res. Commun. 284: 792-797.
Bingle CD and Bingle L (2000). Characterisation of the human plunc gene, a gene product with an upper airways and nasopharyngeal restricted expression pattern. Biochem. Biophys. Acta 1493: 363-367.
Current MPhil/PhD Students
Venkata Satya Dubagunta