The University of Sheffield
Chemical and Biological Engineering

Dr Mark Dickman

Mark DickmanPrincipal Admissions Tutor

Biological and Environmental Systems Group

Tel: +44 (0)114 222 7541
Fax: +44 (0)114 222 7501

email : m.dickman@sheffield.ac.uk

ChELSI Institute
Chemical and Biological Engineering
The University of Sheffield
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD

Research interests

Biography

I obtained a First Class Honours Degree in Biochemistry/Chemistry at the University of Sheffield. I continued at the Krebs Institute, where I obtained my PhD. Following my PhD I joined a biotechnology company, Transgenomic LTD where I worked as a research scientist developing analytical techniques including DNA/RNA Chromatography.

Since joining the Department of Chemical and Biological Engineering at the University of Sheffield, my research has focused on the development and application analytical techniques to study biological systems. In particular, biological mass spectrometry in conjunction with bioseparations have been utilised to study a wide variety of biological systems. Using these analytical approaches we are interested in identifying and characterising protein, protein-RNA/DNA complexes, protein post translational modifications and RNA post transcriptional modifications.

Recent Papers

1RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions.Wiedenheft B, van Duijn E, Bultema J, Waghmare S, Zhou K, Barendregt A, Westphal W, Heck A, Boekema E, Dickman M, Doudna JA. Proc Natl Acad Sci U S A. 2011 May 2. [Epub ahead of print]

Structural basis for CRISPR RNA-guided DNA recognition by Cascade.Jore MM, Lundgren M, van Duijn E, Bultema JB, Westra ER, Waghmare SP, Wiedenheft B, Pul U, Wurm R, Wagner R, Beijer MR, Barendregt A, Zhou K, Snijders AP, Dickman MJ, Doudna JA, Boekema EJ, Heck AJ, van der Oost J, Brouns SJ. Nat Struct Mol Biol. 2011 Apr 3. [Epub ahead of print]

Characterisation and quantification of RNA post-transcriptional modifications using stable isotope labeling of RNA in conjunction with mass spectrometry analysis. Waghmare SP and Dickman MJ. Anal Chem. 2011 Accepted.

Analysis of arginine and lysine methylation utilizing peptide separations at neutral pH and electron transfer dissociation mass spectrometry.Snijders AP, Hung ML, Wilson SA, Dickman MJ. J Am Soc Mass Spectrom. 2010 (1):88-96. Epub 2009 Sep 20.

Studying the mechanism of RNA separations using RNA chromatography and its application in the analysis of ribosomal RNA and RNA:RNA interactions.Waghmare SP, Pousinis P, Hornby DP, Dickman MJ. J Chromatogr A. 2009 Feb 27;1216(9):1377-82. Epub 2008 Dec 30.

Shotgun proteome analysis utilising mixed mode (reversed phase-anion exchange chromatography) in conjunction with reversed phase liquid chromatography mass spectrometry analysis.Phillips HL, Williamson JC, van Elburg KA, Snijders AP, Wright PC, Dickman MJ. Proteomics. 2010 Aug;10(16):2950-60
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Small CRISPR RNAs guide antiviral defense in prokaryotes. Brouns SJ, Jore MM, Lundgren M, Westra ER, Slijkhuis RJ, Snijders AP, Dickman MJ, Makarova KS, Koonin EV, van der Oost J. Science. 2008 Aug 15;321(5891):960-4

Characterization of post-translational modifications of the linker histones H1 and H5 from chicken erythrocytes using mass spectrometry. Snijders AP, Pongdam S, Lambert SJ, Wood CM, Baldwin JP, Dickman MJ. J Proteome Res. 2008 (10):4326-35. Epub 2008 Aug 28.

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Research